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xt index v2 kit  (Illumina Inc)


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    Structured Review

    Illumina Inc xt index v2 kit
    Xt Index V2 Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 3946 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/xt+index+kit/Nextera+XT+Index+Kit/pmc13000721-60-7-11
    Average 99 stars, based on 3946 article reviews
    xt index v2 kit - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Terroir and farming practices drive arbuscular mycorrhizal fungal communities in French vineyards
    Article Snippet: .. The second PCR was performed using a Nextera ® XT Index Kit (Illumina, San Diego, United States) following the manufacturer’s instructions. .. After purification with magnetic beads (AMPure XP), these final PCR products were merged by triplicate , dosed with kit KAPA Library Quantification kit (Roche), multiplexed and sequenced on a MiSeq Illumina sequencer using MiSeq Reagent Kit v3 (600-cycle, Illumina).

    Article Title: In vitro comparative analysis of steamed wood and other lignocellulosic substrates on ruminal fermentation and microbiota
    Article Snippet: Bacterial sequencing was performed via two-step amplification of the V3–V4 regions of the 16S rRNA gene using primers consisting of Illumina overhang adapters and universal bacterial primers (F-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG; R-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC). .. In the second PCR, Illumina sequencing adapters and dual index barcodes were added to the amplicons using a Nextera ® XT Index Kit (Illumina Inc., San Diego, California, USA). ..

    Article Title: Triadic relationships between pasture exposure, gastrointestinal parasites, and hindgut microbiomes in grazing lambs
    Article Snippet: .. During the second PCR, sample-indexing was performed using the Nextera ® XT Index Kit (Illumina, Inc., San Diego, CA, USA), in a 25 μL reaction consisting of 2.5 μL of amplified DNA from the previous PCR, 12.5 μL of PlatinumTM II Hot-Start PCR Master Mix (Thermo Fisher Scientific, Waltham, MA, USA), 1.25 μL of each index, and 10 μL of ultra-pure water. ..

    Article Title: Soil microbial load modulation improves plant–microbe interactions and bioinoculant efficacy in pathogen-stressed soils
    Article Snippet: The PCR amplicons were purified via AMPure XP magnetic beads (Beckman Coulter, Brea, CA, USA). .. A second PCR step was then performed to attach Illumina sequencing adapters and dual indices via the Nextera ® XT Index Kit (Illumina, San Diego, CA, USA) according to the manufacturer’s instructions. ..

    Article Title: High voltage-gated sodium channel gene diversity in Aedes albopictus across Brazil.
    Article Snippet: The quality and concentration of the pooled amplicons (IIS6 + IIIS6 mixture) were analyzed using an Agilent 2100 Bioanalyzer System (Agilent). .. A unique DNA barcode was added to each pool using the Nextera Scientific Reports | (2025) 15:20832 2| https://doi.org/10.1038/s41598-025-08989-x XT index kit (Illumina) in a PCR reaction with the KAPA HiFi HotStart ReadyMix Kit (Roche) according to the manufacturer’s protocol. .. Sequencing was performed on a MiSeq platform (Illumina) at the FIOCRUZ NGS facility using the MiSeq Reagent Kit v2 (500 cycles, paired-end, 2 × 250 bp).

    Article Title: Pregnancy dedifferentiates memory CD8+ T cells into hypofunctional cells with exhaustion-enriched programs.
    Article Snippet: .. 200 live cells per sample/subset were sorted into 96-well optical 2 PCR plates (Thomas Scientific) containing 4μl of lysis buffer at 4°C. cDNA sequencing libraries were 3 generated using Nextera XT DNA Library Prep Kit and Nextera XT Index Kit (Illumina). ..

    Amplification:

    Article Title: Triadic relationships between pasture exposure, gastrointestinal parasites, and hindgut microbiomes in grazing lambs
    Article Snippet: .. During the second PCR, sample-indexing was performed using the Nextera ® XT Index Kit (Illumina, Inc., San Diego, CA, USA), in a 25 μL reaction consisting of 2.5 μL of amplified DNA from the previous PCR, 12.5 μL of PlatinumTM II Hot-Start PCR Master Mix (Thermo Fisher Scientific, Waltham, MA, USA), 1.25 μL of each index, and 10 μL of ultra-pure water. ..

    Hot Start PCR:

    Article Title: Triadic relationships between pasture exposure, gastrointestinal parasites, and hindgut microbiomes in grazing lambs
    Article Snippet: .. During the second PCR, sample-indexing was performed using the Nextera ® XT Index Kit (Illumina, Inc., San Diego, CA, USA), in a 25 μL reaction consisting of 2.5 μL of amplified DNA from the previous PCR, 12.5 μL of PlatinumTM II Hot-Start PCR Master Mix (Thermo Fisher Scientific, Waltham, MA, USA), 1.25 μL of each index, and 10 μL of ultra-pure water. ..

    DNA Library Preparation:

    Article Title: Direct transmission of severe fever with thrombocytopenia syndrome virus from farm-raised fur animals to workers in Weihai, China
    Article Snippet: The PCR products were purified using AMPure XP magnetic beads (Beckman Coulter, Brea, CA, USA). .. Library construction was performed using the Nextera XT DNA Library Preparation Kit and Nextera XT Index Kit (Illumina, San Diego, CA, USA). .. Whole genome sequencing was performed using Illumina Nextseq2000 sequencer.

    Lysis:

    Article Title: Pregnancy dedifferentiates memory CD8+ T cells into hypofunctional cells with exhaustion-enriched programs.
    Article Snippet: .. 200 live cells per sample/subset were sorted into 96-well optical 2 PCR plates (Thomas Scientific) containing 4μl of lysis buffer at 4°C. cDNA sequencing libraries were 3 generated using Nextera XT DNA Library Prep Kit and Nextera XT Index Kit (Illumina). ..

    Sequencing:

    Article Title: Pregnancy dedifferentiates memory CD8+ T cells into hypofunctional cells with exhaustion-enriched programs.
    Article Snippet: .. 200 live cells per sample/subset were sorted into 96-well optical 2 PCR plates (Thomas Scientific) containing 4μl of lysis buffer at 4°C. cDNA sequencing libraries were 3 generated using Nextera XT DNA Library Prep Kit and Nextera XT Index Kit (Illumina). ..

    Generated:

    Article Title: Pregnancy dedifferentiates memory CD8+ T cells into hypofunctional cells with exhaustion-enriched programs.
    Article Snippet: .. 200 live cells per sample/subset were sorted into 96-well optical 2 PCR plates (Thomas Scientific) containing 4μl of lysis buffer at 4°C. cDNA sequencing libraries were 3 generated using Nextera XT DNA Library Prep Kit and Nextera XT Index Kit (Illumina). ..



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